Device
Part:BBa_K317031:Design
Designed by: Yoshiyuki MURAKAMI Group: iGEM10_Tokyo-NoKoGen (2010-10-20)
P(J23109)-RBS(B0034)-pduP_GFP-Term(B0015)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 635
Illegal XhoI site found at 536 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used following 3 primers to construct the fusion protein of N-terminal 18 amino acids of pduP and GFPuv.
pduP-1 : 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAACACTT-3'
pduP-2 : 5'-GAGGAGAAATACTAGATGAACACTTCAGAACTTGAAACCCTTATTCGTAACATTTTGA-3'
pduP-3 : 5'-TGAAAAGTTCTTCTCCTTTACTCATAAGTTGCTCACTCAAAATGTTACGAATAAGGGTTTC-3'